Coding

Part:BBa_K360112:Design

Designed by: Mariana Ruiz Velasco Leyva   Group: iGEM10_UNAM-Genomics_Mexico   (2010-10-14)

Luciferin Regenerating Enzyme (LRE) from Photinus Pyralis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 196
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 402
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 388


Design Notes

None


Source

Originally extracted from Photinus pyralis by Gomi and Kajiyama and engineered for codon optimization and registry standards.

References


  • Gomi, K. and Kajiyama, N. (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin. The Journal of Biological Chemistry, Vol. 276, No. 39.